Describe how an onion peel slide is prepared. Explain the purpose of the safranin, the rinse, the glycerin, and the careful placing of the coverslip.
Hint. Each step exists to solve one specific problem with looking at a transparent, drying-out tissue.
The steps. Wash an onion bulb, cut it vertically, and pull the thin transparent layer from the inner surface of a piece with forceps — this is the onion peel. Place it in a petri dish with a few drops of safranin for 30 seconds, transfer it with a thin brush to a dish of water to rinse, lay it flat on a glass slide without breaking or folding it, add a drop of glycerin, lower a coverslip with a needle so no air bubbles are trapped, wipe off excess glycerin with blotting paper, and observe.
Why each step is there:
| Step | The problem it solves |
|---|---|
| Safranin (red stain), 30 s | The peel is nearly transparent, so its parts have almost no contrast; the stain gives the cells a pinkish colour and makes them visible |
| Rinse in water | Removes excess stain, which would otherwise darken everything and hide the detail the stain was meant to reveal |
| Glycerin | Prevents the cells drying out, and improves clarity |
| Coverslip lowered slowly at an angle | Trapped air bubbles look like dark-edged circles under the microscope and are easily mistaken for cells |
| Blotting paper | Excess glycerin would smear the lens and let the coverslip slide |
Do not break or fold the peel. A folded peel gives two overlapping layers, so you see a confusing double image instead of one clean sheet of cells.
✦ Answer: safranin stains the near-transparent cells so they can be seen, the rinse removes excess stain, glycerin stops the cells drying and improves clarity, and the coverslip is lowered carefully so that no air bubbles are trapped and mistaken for cells.
